Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsResearchers detected a significantly reduced A375 cell viability, an increase in alanine, inositol, nucleotides, and other metabolites that together define apoptosis.
[Scientific Reports]
7753456 {7753456:RKU9VMCS} apa 50 1 162299 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-9fc39a9ce688808e0ec388d4a20dc953%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22RKU9VMCS%22%2C%22library%22%3A%7B%22id%22%3A7753456%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22%5Cu0160pakov%5Cu00e1%20et%20al.%22%2C%22parsedDate%22%3A%222021-05-14%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3E%26%23x160%3Bpakov%26%23xE1%3B%2C%20I.%2C%20Rabajdov%26%23xE1%3B%2C%20M.%2C%20Mi%26%23x10D%3Bkov%26%23xE1%3B%2C%20H.%2C%20Graier%2C%20W.%20F.%2C%20%26amp%3B%20Marekov%26%23xE1%3B%2C%20M.%20%282021%29.%20Effect%20of%20hypoxia%20factors%20gene%20silencing%20on%20ROS%20production%20and%20metabolic%20status%20of%20A375%20malignant%20melanoma%20cells.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%2010325.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-89792-2%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-89792-2%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D7753456%26amp%3Bitem_key%3DRKU9VMCS%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Effect%20of%20hypoxia%20factors%20gene%20silencing%20on%20ROS%20production%20and%20metabolic%20status%20of%20A375%20malignant%20melanoma%20cells%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ivana%22%2C%22lastName%22%3A%22%5Cu0160pakov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Miroslava%22%2C%22lastName%22%3A%22Rabajdov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Helena%22%2C%22lastName%22%3A%22Mi%5Cu010dkov%5Cu00e1%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wolfgang%20F.%22%2C%22lastName%22%3A%22Graier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22M%5Cu00e1ria%22%2C%22lastName%22%3A%22Marekov%5Cu00e1%22%7D%5D%2C%22abstractNote%22%3A%22The%20innate%20response%20of%20melanocytes%20to%20exogenous%20or%20endogenous%20stress%20stimuli%20like%20extreme%20pH%20and%20temperature%2C%20metabolite%20and%20oxygen%20deficiency%20or%20a%20high%20UV%20dose%20initiates%20a%20cellular%20stress%20response.%20This%20process%20activates%20adaptive%20processes%20to%20minimize%20the%20negative%20impact%20of%20the%20stressor%20on%20the%20pigment%20cell.%20Under%20physiological%20conditions%2C%20a%20non-cancer%20cell%20is%20directed%20to%20apoptosis%20if%20the%20stressor%20persists.%20However%2C%20malignant%20melanoma%20cells%20will%20survive%20persistent%20stress%20thanks%20to%20distinct%20%5C%22cancerous%5C%22%20signaling%20pathways%20%28e.g.%20MEK%29%20and%20transcription%20factors%20that%20regulate%20the%20expression%20of%20so-called%20%5C%22survival%20genes%5C%22%20%28e.g.%20HIF%2C%20MITF%29.%20In%20this%20survival%20response%20of%20cancer%20cells%2C%20MEK%20pathway%20directs%20melanoma%20cells%20to%20deregulate%20mitochondrial%20metabolism%2C%20to%20accumulate%20reduced%20species%20%28NADH%29%2C%20and%20to%20centralize%20metabolism%20in%20the%20cytosol.%20The%20aim%20of%20this%20work%20was%20to%20study%20the%20effect%20of%20gene%20silencing%20in%20malignant%20melanoma%20A375%20cells%20on%20metabolic%20processes%20in%20cytosol%20and%20mitochondria.%20Gene%20silencing%20of%20HIF-1%5Cu03b1%2C%20and%20miR-210%20in%20normoxia%20and%20pseudohypoxia%2C%20and%20analysis%20of%20its%20effect%20on%20MITF-M%2C%20and%20PDHA1%20expression.%20Detection%20of%20cytosolic%20NADH%20by%20Peredox-mCherry%20Assay.%20Detection%20of%20OCR%2C%20and%20ECAR%20using%20Seahorse%20XF96.%20Measurement%20of%20produced%20O2%5Cu2022%5Cu2212%20with%20MitoTracker%20Red%20CMXRos.%201H%20NMR%20analysis%20of%20metabolites%20present%20in%20cell%20suspension%2C%20and%20medium.%20By%20gene%20silencing%20of%20HIF-1%5Cu03b1%20and%20miR-210%20the%20expression%20of%20PDHA1%20was%20upregulated%20while%20that%20of%20MITF-M%20was%20downregulated%2C%20yielding%20acceleration%20of%20mitochondrial%20respiratory%20activity%20and%20thus%20elimination%20of%20ROS.%20Hence%2C%20we%20detected%20a%20significantly%20reduced%20A375%20cell%20viability%2C%20an%20increase%20in%20alanine%2C%20inositol%2C%20nucleotides%2C%20and%20other%20metabolites%20that%20together%20define%20apoptosis.%20Based%20on%20the%20results%20of%20measurements%20of%20mitochondrial%20resipiratory%20activity%2C%20ROS%20production%2C%20and%20changes%20in%20the%20metabolites%20obtained%20in%20cells%20under%20the%20observed%20conditions%2C%20we%20concluded%20that%20silencing%20of%20HIF-1%5Cu03b1%20and%20miR-210%20yields%20apoptosis%20and%2C%20ultimately%2C%20apoptotic%20cell%20death%20in%20A375%20melanoma%20cells.%22%2C%22date%22%3A%222021-05-14%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-89792-2%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-021-89792-2%22%2C%22deleted%22%3A1%2C%22collections%22%3A%5B%22DUPVZUHA%22%5D%2C%22dateModified%22%3A%222021-05-14T17%3A44%3A37Z%22%7D%7D%5D%7D
Špaková, I., Rabajdová, M., Mičková, H., Graier, W. F., & Mareková, M. (2021). Effect of hypoxia factors gene silencing on ROS production and metabolic status of A375 malignant melanoma cells. Scientific Reports, 11(1), 10325. https://doi.org/10.1038/s41598-021-89792-2 Cite
