Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsReal-time cell migration analysis, measuring wound closure rates over 24 hours, revealed that 18αGA essentially blocked wound closure whereas hexokinase and GSK-7975A showed relatively minimal effects.
[Journal of Cellular Physiology]
7753456 Q44X7XR9 items 1 apa 0 default asc 1 164001 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-362463ea6bdcddbeb558d31b5bcf10e8%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22Q44X7XR9%22%2C%22library%22%3A%7B%22id%22%3A7753456%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Hudson%20et%20al.%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EHudson%2C%20L.%2C%20Begg%2C%20M.%2C%20Wright%2C%20B.%2C%20Cheek%2C%20T.%2C%20Jahoda%2C%20C.%20A.%20B.%2C%20%26amp%3B%20Reynolds%2C%20N.%20J.%20%28n.d.%29.%20Dominant%20effect%20of%20gap%20junction%20communication%20in%20wound-induced%20calcium-wave%2C%20NFAT%20activation%20and%20wound%20closure%20in%20keratinocytes.%20%3Ci%3EJournal%20of%20Cellular%20Physiology%3C%5C%2Fi%3E%2C%20%3Ci%3En%5C%2Fa%3C%5C%2Fi%3E%28n%5C%2Fa%29.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fjcp.30488%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fjcp.30488%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D7753456%26amp%3Bitem_key%3DQ44X7XR9%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Dominant%20effect%20of%20gap%20junction%20communication%20in%20wound-induced%20calcium-wave%2C%20NFAT%20activation%20and%20wound%20closure%20in%20keratinocytes%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Laura%22%2C%22lastName%22%3A%22Hudson%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Malcolm%22%2C%22lastName%22%3A%22Begg%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Blythe%22%2C%22lastName%22%3A%22Wright%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tim%22%2C%22lastName%22%3A%22Cheek%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Colin%20A.%20B.%22%2C%22lastName%22%3A%22Jahoda%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nick%20J.%22%2C%22lastName%22%3A%22Reynolds%22%7D%5D%2C%22abstractNote%22%3A%22Wounding%20induces%20a%20calcium%20wave%20and%20disrupts%20the%20calcium%20gradient%20across%20the%20epidermis%20but%20mechanisms%20mediating%20calcium%20and%20downstream%20signalling%2C%20and%20longer-term%20wound%20healing%20responses%20are%20incompletely%20understood.%20As%20expected%2C%20live-cell%20confocal%20imaging%20of%20Fluo-4-loaded%20normal%20human%20keratinocytes%20showed%20an%20immediate%20increase%20in%20%5BCa2%2B%5Di%20at%20the%20wound%20edge%20that%20spread%20as%20a%20calcium%20wave%20%288.3%20%5Cu00b5m%5C%2Fs%29%20away%20from%20the%20wound%20edge%20with%20gradually%20diminishing%20rate%20of%20rise%20and%20amplitude.%20The%20amplitude%20and%20area%20under%20the%20curve%20of%20%5BCa2%2B%5Di%20flux%20was%20increased%20in%20high%20%281.2%20mM%29%20%5BCa2%2B%5Do%20media.%2018%5Cu03b1-glycyrrhetinic%20acid%20%2818%5Cu03b1GA%29%2C%20a%20gap-junction%20inhibitor%20or%20hexokinase%2C%20an%20ATP%20scavenger%2C%20blocked%20the%20wound-induced%20calcium%20wave%2C%20dependent%20in%20part%20on%20%5BCa2%2B%5Do.%20Wounding%20in%20a%20high%20%5BCa2%2B%5Do%20increased%20nuclear%20factor%20of%20activated%20T-cells%20%28NFAT%29%20but%20not%20NFkB%20activation%2C%20assessed%20by%20dual-luciferase%20receptor%20assays%20compared%20to%20unwounded%20cells.%20Treatment%20with%2018%5Cu03b1GA%20or%20the%20store-operated%20channel%20blocker%20GSK-7975A%20inhibited%20wound-induced%20NFAT%20activation%2C%20whereas%20treatment%20with%20hexokinase%20did%20not.%20Real-time%20cell%20migration%20analysis%2C%20measuring%20wound%20closure%20rates%20over%2024%20h%2C%20revealed%20that%2018%5Cu03b1GA%20essentially%20blocked%20wound%20closure%20whereas%20hexokinase%20and%20GSK-7975A%20showed%20relatively%20minimal%20effects.%20Together%20these%20data%20indicate%20that%20while%20both%20gap-junction%20communication%20and%20ATP%20release%20from%20damaged%20cells%20are%20important%20in%20regulating%20the%20wound-induced%20calcium%20wave%2C%20long-term%20transcriptional%20and%20functional%20responses%20are%20dominantly%20regulated%20by%20gap-junction%20communication.%22%2C%22date%22%3A%22%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1002%5C%2Fjcp.30488%22%2C%22ISSN%22%3A%221097-4652%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fonlinelibrary.wiley.com%5C%2Fdoi%5C%2Fabs%5C%2F10.1002%5C%2Fjcp.30488%22%2C%22deleted%22%3A1%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-06-28T17%3A34%3A39Z%22%7D%7D%5D%7D
Hudson, L., Begg, M., Wright, B., Cheek, T., Jahoda, C. A. B., & Reynolds, N. J. (n.d.). Dominant effect of gap junction communication in wound-induced calcium-wave, NFAT activation and wound closure in keratinocytes. Journal of Cellular Physiology, n/a(n/a). https://doi.org/10.1002/jcp.30488 Cite
