liquid chromatography

[Translational Psychiatry] Utilizing iPSCs from 25 human donors, researchers generated 3D cerebral organoids, employed 16-plex isobaric sample-barcoding chemistry, and simultaneously subjected samples to comprehensive high-throughput liquid-chromatography/mass-spectrometry quantitative proteomics.

[Molecular Metabolism] Human duodenal organoids were CRISPR-Cas9 modified to express the fluorescent protein Venus or the Ca2+ sensor GCaMP7s under control of the endogenous motilin promoter.

[Scientific Reports] Researchers profiled end-induction plasma, marrow, and cerebrospinal fluid (CSF) from ten children with b-cell acute lymphoblastic leukemia using liquid chromatography-mass spectrometry and observed plasma-marrow correlations for metabolites in pathways associated with end-induction residual disease and plasma-CSF correlations for a biomarker of fatigue.

[Natural Product Research] The effects of Larrea divaricata, polyphenols and quercetin-3-methyl ether were assessed on B16F10 melanoma cells by determining the proliferation, viability, apoptosis induction and the expression and phosphorylation of STAT3.

[Scientific Reports] Researchers performed untargeted ultra-high performance liquid chromatography coupled to high-resolution mass spectrometry-based polar metabolomics and lipidomics to non-transformed and transformed colon cell lines as well as tissue samples from ten colorectal cancer patients.

[Naunyn-Schmiedebergs Archives of Pharmacology] Scientists investigated the effect of diclofenac, a selective COX-2 inhibitor, on cell adhesion molecules and apoptosis in human colon adenocarcinoma cells. Levels of homing cell adhesion molecule, intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and epithelial cell adhesion molecule were evaluated in cancer cells overexpressing or not expressing COX-2.