Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsScientists describe a protocol to generate cardiomyocytes, cardiac fibroblasts and cardiac endothelial cells, the three principal cell types in the heart, from human induced pluripotent stem cells and combine them in 3D cardiac microtissues.
[Nature Protocols]
6445218 {6445218:5TFJMMNC} apa 50 1 160577 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-1854032846421216c844016f83244803%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%225TFJMMNC%22%2C%22library%22%3A%7B%22id%22%3A6445218%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Campostrini%20et%20al.%22%2C%22parsedDate%22%3A%222021-03-26%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ECampostrini%2C%20G.%2C%20Meraviglia%2C%20V.%2C%20Giacomelli%2C%20E.%2C%20van%20Helden%2C%20R.%20W.%20J.%2C%20Yiangou%2C%20L.%2C%20Davis%2C%20R.%20P.%2C%20Bellin%2C%20M.%2C%20Orlova%2C%20V.%20V.%2C%20%26amp%3B%20Mummery%2C%20C.%20L.%20%282021%29.%20Generation%2C%20functional%20analysis%20and%20applications%20of%20isogenic%20three-dimensional%20self-aggregating%20cardiac%20microtissues%20from%20human%20pluripotent%20stem%20cells.%20%3Ci%3ENature%20Protocols%3C%5C%2Fi%3E%2C%201%26%23x2013%3B44.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41596-021-00497-2%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41596-021-00497-2%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445218%26amp%3Bitem_key%3D5TFJMMNC%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Generation%2C%20functional%20analysis%20and%20applications%20of%20isogenic%20three-dimensional%20self-aggregating%20cardiac%20microtissues%20from%20human%20pluripotent%20stem%20cells%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Giulia%22%2C%22lastName%22%3A%22Campostrini%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Viviana%22%2C%22lastName%22%3A%22Meraviglia%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisa%22%2C%22lastName%22%3A%22Giacomelli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ruben%20W.%20J.%22%2C%22lastName%22%3A%22van%20Helden%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Loukia%22%2C%22lastName%22%3A%22Yiangou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Richard%20P.%22%2C%22lastName%22%3A%22Davis%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Milena%22%2C%22lastName%22%3A%22Bellin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Valeria%20V.%22%2C%22lastName%22%3A%22Orlova%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christine%20L.%22%2C%22lastName%22%3A%22Mummery%22%7D%5D%2C%22abstractNote%22%3A%22Tissue-like%20structures%20from%20human%20pluripotent%20stem%20cells%20containing%20multiple%20cell%20types%20are%20transforming%20our%20ability%20to%20model%20and%20understand%20human%20development%20and%20disease.%20Here%20we%20describe%20a%20protocol%20to%20generate%20cardiomyocytes%20%28CMs%29%2C%20cardiac%20fibroblasts%20%28CFs%29%20and%20cardiac%20endothelial%20cells%20%28ECs%29%2C%20the%20three%20principal%20cell%20types%20in%20the%20heart%2C%20from%20human%20induced%20pluripotent%20stem%20cells%20%28hiPSCs%29%20and%20combine%20them%20in%20three-dimensional%20%283D%29%20cardiac%20microtissues%20%28MTs%29.%20We%20include%20details%20of%20how%20to%20differentiate%2C%20isolate%2C%20cryopreserve%20and%20thaw%20the%20component%20cells%20and%20how%20to%20construct%20and%20analyze%20the%20MTs.%20The%20protocol%20supports%20hiPSC-CM%20maturation%20and%20allows%20replacement%20of%20one%20or%20more%20of%20the%20three%20heart%20cell%20types%20in%20the%20MTs%20with%20isogenic%20variants%20bearing%20disease%20mutations.%20Differentiation%20of%20each%20cell%20type%20takes%20~30%20d%2C%20while%20MT%20formation%20and%20maturation%20requires%20another%2020%20d.%20No%20specialist%20equipment%20is%20needed%20and%20the%20method%20is%20inexpensive%2C%20requiring%20just%205%2C000%20cells%20per%20MT.%22%2C%22date%22%3A%222021-03-26%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41596-021-00497-2%22%2C%22ISSN%22%3A%221750-2799%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41596-021-00497-2%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-03-26T22%3A24%3A01Z%22%7D%7D%5D%7D
Campostrini, G., Meraviglia, V., Giacomelli, E., van Helden, R. W. J., Yiangou, L., Davis, R. P., Bellin, M., Orlova, V. V., & Mummery, C. L. (2021). Generation, functional analysis and applications of isogenic three-dimensional self-aggregating cardiac microtissues from human pluripotent stem cells. Nature Protocols, 1–44. https://doi.org/10.1038/s41596-021-00497-2 Cite
