In Vitro Model of Ischemic Heart Failure Using Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

By
Justin.choi
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hiPSCs were differentiated to functional hiPSC derived cardiomyocytes (hiPSC-CMs) and then purified using either a simulated ischemia media or by using magnetic antibody-based purification targeting the non-myocyte population for depletion from the cell population. Flow cytometry analysis confirmed that each purification approach generated hiPSC-CM cultures of >94% cTnT+ cells.
[JCI Insight]
Davis, J., Chouman, A., Creech, J., Rocha, A. M. da, Ponce-Balbuena, D., Vazquez, E. N. J., Nichols, R., Lozhkin, A., Madamanchi, N. R., Campbell, K. F., & Herron, T. J. (2021). In vitro model of ischemic heart failure using human induced pluripotent stem cell-derived cardiomyocytes. JCI Insight. https://doi.org/10.1172/jci.insight.134368 Cite
Abstract