Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsInvestigators analyzed Lgr5-Cre; APCmin; HER2V777LTg mice by morphologic and gene expression assays on intestinal sections and organoids derived from the epithelium.
[Cellular and Molecular Gastroenterology and Hepatology]
6445218 E4PWV5UE items 1 apa 0 default asc 1 161662 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-b6147775cf2a0835333d94fc7a6a2647%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22E4PWV5UE%22%2C%22library%22%3A%7B%22id%22%3A6445218%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Murray%20et%20al.%22%2C%22parsedDate%22%3A%222021-04-27%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMurray%2C%20E.%2C%20Cheng%2C%20X.%2C%20Krishna%2C%20A.%2C%20Jin%2C%20X.%2C%20Ohara%2C%20T.%20E.%2C%20Stappenbeck%2C%20T.%20S.%2C%20%26amp%3B%20Bose%2C%20R.%20%282021%29.%20HER2%20and%20APC%20mutations%20promote%20altered%20crypt-villus%20morphology%20and%20marked%20hyperplasia%20in%20the%20intestinal%20epithelium.%20%3Ci%3ECellular%20and%20Molecular%20Gastroenterology%20and%20Hepatology%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jcmgh.2021.04.012%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jcmgh.2021.04.012%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445218%26amp%3Bitem_key%3DE4PWV5UE%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22HER2%20and%20APC%20mutations%20promote%20altered%20crypt-villus%20morphology%20and%20marked%20hyperplasia%20in%20the%20intestinal%20epithelium%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elisa%22%2C%22lastName%22%3A%22Murray%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xiaoqing%22%2C%22lastName%22%3A%22Cheng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anagha%22%2C%22lastName%22%3A%22Krishna%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xiaohua%22%2C%22lastName%22%3A%22Jin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Takahiro%20E.%22%2C%22lastName%22%3A%22Ohara%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Thaddeus%20S.%22%2C%22lastName%22%3A%22Stappenbeck%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ron%22%2C%22lastName%22%3A%22Bose%22%7D%5D%2C%22abstractNote%22%3A%22Background%20%26%20Aims%5CnThe%20Cancer%20Genome%20Atlas%20project%20%28TCGA%29%20has%20identified%20HER2%20mutations%20or%20amplification%20in%207%25%20of%20colon%20cancers.%20In%20addition%20to%20HER2%20mutations%2C%20colon%20cancer%20patients%20also%20possess%20co-occurring%20mutations%20in%20genes%20such%20as%20APC.%20Here%2C%20we%20investigated%20the%20role%20of%20HER2%20and%20APC%20mutations%20on%20the%20crypt-villus%20architecture%20of%20the%20intestinal%20epithelium%2C%20localization%20of%20secretory%20cells%2C%20and%20expression%20of%20intestinal%20stem%20cell%20markers.%5CnMethods%5CnWe%20generated%20a%20HER2%20transgenic%20mouse%20%28HER2V777L%20Tg%29%20possessing%20an%20activating%20mutation%20commonly%20found%20in%20colorectal%20cancer%20patients%2C%20HER2V777L%2C%20using%20TALEN%20based%20gene%20editing%20technology.%20We%20expressed%20the%20HER2V777L%20transgene%20in%20mouse%20small%20intestine%20and%20colon%20using%20Lgr5-Cre%20and%20Villin-Cre%20recombinases.%20In%20addition%2C%20we%20analyzed%20Lgr5-Cre%3B%20APCmin%3B%20HER2V777L%20Tg%20mice%20by%20morphologic%20and%20gene%20expression%20assays%20on%20intestinal%20sections%20and%20organoids%20derived%20from%20the%20epithelium.%5CnResults%5CnHER2V777L%20expression%20resulted%20in%20hypertrophic%20crypt%20formation%20with%20expanded%20zones%20of%20proliferation.%20Proximal%20intestinal%20villi%20showed%20increased%20abundance%20of%20multiple%20differentiated%20lineages%20including%20extensive%20intermediate%20cell%20differentiation%2C%20as%20evidenced%20by%20MUC2%5C%2FMMP7%20co-immunofluorescence%20and%20transmission%20electron%20microscopy.%20HER2V777L%20expression%20in%20the%20context%20of%20APC%20loss%20resulted%20in%20further%20enhancement%20and%20expansion%20of%20the%20proliferative%20crypt%20compartment.%5CnConclusions%5CnWe%20established%20an%20epithelial%20intrinsic%20role%20for%20HER2V777L%20on%20enhanced%20cellular%20proliferation.%20Additionally%2C%20we%20determined%20that%20HER2%20and%20APC%20mutations%2C%20when%20combined%2C%20promote%20enhanced%20proliferation%20of%20intestinal%20crypts.%22%2C%22date%22%3A%22April%2027%2C%202021%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.jcmgh.2021.04.012%22%2C%22ISSN%22%3A%222352-345X%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.sciencedirect.com%5C%2Fscience%5C%2Farticle%5C%2Fpii%5C%2FS2352345X21000837%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-04-28T04%3A17%3A06Z%22%7D%7D%5D%7D
Murray, E., Cheng, X., Krishna, A., Jin, X., Ohara, T. E., Stappenbeck, T. S., & Bose, R. (2021). HER2 and APC mutations promote altered crypt-villus morphology and marked hyperplasia in the intestinal epithelium. Cellular and Molecular Gastroenterology and Hepatology. https://doi.org/10.1016/j.jcmgh.2021.04.012 Cite
