Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsIn vitro, epithelial-mesenchymal transition (EMT) was induced by the administration of transforming growth factor-β1 in a human alveolar epithelial cell line.
[Laboratory Investigation]
7753456 RT2ET24M items 1 apa 0 default asc 1 163830 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-cdd19e2a73b60a7670128f3a856c3d7e%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22RT2ET24M%22%2C%22library%22%3A%7B%22id%22%3A7753456%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Chen%20et%20al.%22%2C%22parsedDate%22%3A%222021-06-24%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EChen%2C%20L.%2C%20Alam%2C%20A.%2C%20Pac-Soo%2C%20A.%2C%20Chen%2C%20Q.%2C%20Shang%2C%20Y.%2C%20Zhao%2C%20H.%2C%20Yao%2C%20S.%2C%20%26amp%3B%20Ma%2C%20D.%20%282021%29.%20Pretreatment%20with%20valproic%20acid%20alleviates%20pulmonary%20fibrosis%20through%20epithelial%26%23x2013%3Bmesenchymal%20transition%20inhibition%20in%20vitro%20and%20in%20vivo.%20%3Ci%3ELaboratory%20Investigation%3C%5C%2Fi%3E%2C%201%26%23x2013%3B10.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41374-021-00617-2%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41374-021-00617-2%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D7753456%26amp%3Bitem_key%3DRT2ET24M%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Pretreatment%20with%20valproic%20acid%20alleviates%20pulmonary%20fibrosis%20through%20epithelial%5Cu2013mesenchymal%20transition%20inhibition%20in%20vitro%20and%20in%20vivo%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lin%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Azeem%22%2C%22lastName%22%3A%22Alam%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Aurelie%22%2C%22lastName%22%3A%22Pac-Soo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Qian%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22You%22%2C%22lastName%22%3A%22Shang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hailin%22%2C%22lastName%22%3A%22Zhao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shanglong%22%2C%22lastName%22%3A%22Yao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Daqing%22%2C%22lastName%22%3A%22Ma%22%7D%5D%2C%22abstractNote%22%3A%22Epithelial%5Cu2013mesenchymal%20transition%20%28EMT%29%20plays%20a%20crucial%20role%20in%20the%20development%20of%20pulmonary%20fibrosis.%20This%20study%20aims%20to%20investigate%20the%20effects%20of%20valproic%20acid%20%28VPA%29%20on%20EMT%20in%20vitro%20and%20in%20vivo.%20In%20vitro%2C%20EMT%20was%20induced%20by%20the%20administration%20of%20transforming%20growth%20factor-%5Cu03b21%20%28TGF-%5Cu03b21%29%20in%20a%20human%20alveolar%20epithelial%20cell%20line%20%28A549%29.%20The%20dose%20effects%20of%20VPA%20%280.1%5Cu20133%5Cu2009mM%29%20on%20EMT%20were%20subsequently%20evaluated%20at%20different%20timepoints.%20VPA%20%281%5Cu2009mM%29%20was%20applied%20prior%20to%20the%20administration%20of%20TGF-%5Cu03b21%20and%20the%20expression%20of%20E-cadherin%2C%20vimentin%2C%20p-Smad2%5C%2F3%20and%20p-Akt%20was%20assessed.%20In%20addition%2C%20the%20effects%20of%20a%20TGF-%5Cu03b2%20type%20I%20receptor%20inhibitor%20%28A8301%29%20and%20PI3K-Akt%20inhibitor%20%28LY294002%29%20on%20EMT%20were%20evaluated.%20In%20vivo%2C%20the%20effects%20of%20VPA%20on%20bleomycin-induced%20lung%20fibrosis%20were%20evaluated%20by%20assessing%20variables%20such%20as%20survival%20rate%2C%20body%20weight%20and%20histopathological%20changes%2C%20whilst%20the%20expression%20of%20E-cadherin%20and%20vimentin%20in%20lung%20tissue%20was%20also%20evaluated.%20A8301%20and%20LY294002%20were%20used%20to%20ascertain%20the%20cellular%20signaling%20pathways%20involved%20in%20this%20model.%20The%20administration%20of%20VPA%20prior%20to%20TGF-%5Cu03b21%20in%20A549%20cells%20prevented%20EMT%20in%20both%20a%20time-%20and%20concentration-dependent%20manner.%20Pretreatment%20with%20VPA%20downregulated%20the%20expression%20of%20both%20p-Smad2%5C%2F3%20and%20p-Akt.%20A8301%20administration%20increased%20the%20expression%20of%20E-cadherin%20and%20reduced%20the%20expression%20of%20vimentin.%20LY294002%20inhibited%20Akt%20phosphorylation%20induced%20by%20TGF-%5Cu03b21%20but%20failed%20to%20prevent%20EMT.%20Pretreatment%20with%20VPA%20both%20increased%20the%20survival%20rate%20and%20prevented%20the%20loss%20of%20body%20weight%20in%20mice%20with%20pulmonary%20fibrosis.%20Interestingly%2C%20both%20VPA%20and%20A8301%20prevented%20EMT%20and%20facilitated%20an%20improvement%20in%20lung%20structure.%20Overall%2C%20pretreatment%20with%20VPA%20attenuated%20the%20development%20of%20pulmonary%20fibrosis%20by%20inhibiting%20EMT%20in%20mice%2C%20which%20was%20associated%20with%20Smad2%5C%2F3%20deactivation%20but%20without%20Akt%20cellular%20signal%20involvement.%22%2C%22date%22%3A%222021-06-24%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41374-021-00617-2%22%2C%22ISSN%22%3A%221530-0307%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41374-021-00617-2%22%2C%22collections%22%3A%5B%2243ZBJFKZ%22%5D%2C%22dateModified%22%3A%222021-06-24T17%3A07%3A58Z%22%7D%7D%5D%7D
Chen, L., Alam, A., Pac-Soo, A., Chen, Q., Shang, Y., Zhao, H., Yao, S., & Ma, D. (2021). Pretreatment with valproic acid alleviates pulmonary fibrosis through epithelial–mesenchymal transition inhibition in vitro and in vivo. Laboratory Investigation, 1–10. https://doi.org/10.1038/s41374-021-00617-2 Cite
