The Evidence of a Macrophage Barrier in the Xenotransplantation of Human Hematopoietic Stem Cells to Severely Immunodeficient Rats

The authors generated a rat xenotransplantation model to prove the role of macrophages as a xenotransplantation barrier. Immunodeficiency in SRG rats, which were Sprague–Dawley rats lacking Rag2 and Il2rg, was confirmed by flow cytometry and spleen immunostaining.