Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsSingle cell RNA sequencing of neonatal human and mouse lung was used to identify the heterogeneity of pulmonary endothelial progenitor cells (EPCs). CRISPR/Cas9 gene editing was used to genetically label and purify mouse pulmonary EPCs.
[American Journal of Respiratory and Critical Care Medicine]
6445212 {6445212:TIIM323V} apa 50 1 160031 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-5eb38966bd63b473a3142bd683d3ee54%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22TIIM323V%22%2C%22library%22%3A%7B%22id%22%3A6445212%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Wang%20et%20al.%22%2C%22parsedDate%22%3A%222021-03-11%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EWang%2C%20G.%2C%20Wen%2C%20B.%2C%20Ren%2C%20X.%2C%20Li%2C%20E.%2C%20Zhang%2C%20Y.%2C%20Guo%2C%20M.%2C%20Xu%2C%20Y.%2C%20Whitsett%2C%20J.%20A.%2C%20Kalin%2C%20T.%20V.%2C%20%26amp%3B%20Kalinichenko%2C%20V.%20V.%20%282021%29.%20Generation%20of%20Pulmonary%20Endothelial%20Progenitor%20Cells%20for%20Cell-Based%20Therapy%20Using%20Interspecies%20Mouse-Rat%20Chimeras.%20%3Ci%3EAmerican%20Journal%20of%20Respiratory%20and%20Critical%20Care%20Medicine%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1164%5C%2Frccm.202003-0758OC%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1164%5C%2Frccm.202003-0758OC%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445212%26amp%3Bitem_key%3DTIIM323V%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Generation%20of%20Pulmonary%20Endothelial%20Progenitor%20Cells%20for%20Cell-Based%20Therapy%20Using%20Interspecies%20Mouse-Rat%20Chimeras%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Guolun%22%2C%22lastName%22%3A%22Wang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Bingqiang%22%2C%22lastName%22%3A%22Wen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xiaomeng%22%2C%22lastName%22%3A%22Ren%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Enhong%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yufang%22%2C%22lastName%22%3A%22Zhang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Minzhe%22%2C%22lastName%22%3A%22Guo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yan%22%2C%22lastName%22%3A%22Xu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jeffrey%20A%22%2C%22lastName%22%3A%22Whitsett%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tanya%20V.%22%2C%22lastName%22%3A%22Kalin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Vladimir%20V.%22%2C%22lastName%22%3A%22Kalinichenko%22%7D%5D%2C%22abstractNote%22%3A%22Rationale%3A%20While%20pulmonary%20endothelial%20progenitor%20cells%20%28EPCs%29%20hold%20promise%20for%20cell-based%20therapies%20for%20neonatal%20pulmonary%20disorders%2C%20whether%20EPCs%20can%20be%20derived%20from%20pluripotent%20embryonic%20stem%20cells%20%28ESCs%29%20or%20induced%20pluripotent%20stem%20cells%20%28iPSCs%29%20remains%20unknown.%20Objective%3A%20To%20investigate%20heterogeneity%20of%20pulmonary%20EPCs%20and%20derive%20functional%20EPCs%20from%20pluripotent%20ESCs.%20%20%20Methods%3A%20Single%20cell%20RNA%20sequencing%20of%20neonatal%20human%20and%20mouse%20lung%20was%20used%20to%20identify%20the%20heterogeneity%20of%20pulmonary%20EPCs.%20CRISPR%5C%2FCas9%20gene%20editing%20was%20used%20to%20genetically%20label%20and%20purify%20mouse%20pulmonary%20EPCs.%20Functional%20properties%20of%20the%20EPCs%20were%20assessed%20after%20cell%20transplantation%20into%20neonatal%20mice%20with%20S52F%20Foxf1%20mutation%2C%20a%20mouse%20model%20of%20alveolar%20capillary%20dysplasia%20with%20misalignment%20of%20pulmonary%20veins%20%28ACDMPV%29.%20Interspecies%20mouse-rat%20chimeras%20were%20produced%20through%20blastocyst%20complementation%20to%20generate%20EPCs%20from%20pluripotent%20ESCs%20for%20cell%20therapy%20in%20ACDMPV%20mice.%20Measurements%20and%20Main%20Results%3A%20We%20identified%20a%20unique%20population%20of%20EPCs%2C%20FOXF1%2BcKIT%2B%20EPCs%2C%20as%20a%20subset%20of%20recently%20described%20general%20capillary%20cells%20%28gCAPs%29%20expressing%20SMAD7%2C%20ZBTB20%2C%20NFIA%2C%20DLL4%2C%20but%20lacking%20mature%20arterial%2C%20venous%20and%20lymphatic%20markers.%20FOXF1%2BcKIT%2B%20gCAPs%20are%20reduced%20in%20ACDMPV%20and%20their%20transcriptomic%20signature%20is%20conserved%20in%20mouse%20and%20human%20lungs.%20After%20cell%20transplantation%20into%20the%20neonatal%20circulation%20of%20ACDMPV%20mice%2C%20FOXF1%2BcKIT%2B%20gCAPs%20engraft%20into%20the%20pulmonary%20vasculature%2C%20stimulate%20angiogenesis%2C%20improve%20oxygenation%20and%20prevent%20alveolar%20simplification.%20FOXF1%2BcKIT%2B%20gCAPs%2C%20produced%20from%20ESCs%20in%20interspecies%20chimeras%2C%20are%20fully%20competent%20to%20stimulate%20neonatal%20lung%20angiogenesis%20and%20alveolarization%20in%20ACDMPV%20mice.%20Conclusions%3A%20Cell-based%20therapy%20using%20donor%20or%20ESC%5C%2FiPSC-derived%20FOXF1%2BcKIT%2B%20endothelial%20progenitors%20may%20be%20considered%20for%20treatment%20of%20human%20ACDMPV.%22%2C%22date%22%3A%22March%2011%2C%202021%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1164%5C%2Frccm.202003-0758OC%22%2C%22ISSN%22%3A%221073-449X%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.atsjournals.org%5C%2Fdoi%5C%2F10.1164%5C%2Frccm.202003-0758OC%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-03-12T17%3A38%3A54Z%22%7D%7D%5D%7D
Wang, G., Wen, B., Ren, X., Li, E., Zhang, Y., Guo, M., Xu, Y., Whitsett, J. A., Kalin, T. V., & Kalinichenko, V. V. (2021). Generation of Pulmonary Endothelial Progenitor Cells for Cell-Based Therapy Using Interspecies Mouse-Rat Chimeras. American Journal of Respiratory and Critical Care Medicine. https://doi.org/10.1164/rccm.202003-0758OC Cite
