Evaluation of Tissue Stem Cell Derived Human Intestinal Organoids, a Physiologically Relevant Model to Evaluate Cytochrome P450 Induction in Gut

Matched human enteroid and colonoid lines, generated from ileal and colon biopsies from two donors, were cultured in extracellular matrix for three days, followed by a single 48h treatment with rifampin, omeprazole, CITCO and phenytoin at concentrations that induce target genes in hepatocytes.