The authors generated a Zika virus mutant, ZE4B-36, which combined mutations in both E glycosylation and NS4B P36.
[npj Vaccines]
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Adam, A., Fontes-Garfias, C. R., Sarathy, V. V., Liu, Y., Luo, H., Davis, E., Li, W., Muruato, A. E., Wang, B., Ahatov, R., Mahmoud, Y., Shan, C., Osman, S. R., Widen, S. G., Barrett, A. D. T., Shi, P.-Y., & Wang, T. (2021). A genetically stable Zika virus vaccine candidate protects mice against virus infection and vertical transmission. Npj Vaccines, 6(1), 1–11. https://doi.org/10.1038/s41541-021-00288-6 Cite