Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsColony formation assay and flow cytometry were used to evaluate the effect of garcinone C on the proliferation and cell cycle progression of colon cancer cells.
[Phytomedicine]
6807162 {6807162:V4UG8R6N} apa 50 1 153722 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-d84db2ba039868695629b8967e8d26ba%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22V4UG8R6N%22%2C%22library%22%3A%7B%22id%22%3A6807162%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Chen%20et%20al.%22%2C%22parsedDate%22%3A%222020-09-03%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EChen%2C%20J.%2C%20Qiu%2C%20S.%2C%20Kim%2C%20J.%20T.%2C%20Cho%2C%20J.%20S.%2C%20Moon%2C%20J.%20H.%2C%20Zhou%2C%20Y.%2C%20Auh%2C%20J.-H.%2C%20%26amp%3B%20Lee%2C%20H.%20J.%20%282020%29.%20Garcinone%20C%20Suppresses%20Colon%20Tumorigenesis%20Through%20the%20Gli1-dependent%20Hedgehog%20Signaling%20Pathway.%20%3Ci%3EPhytomedicine%3C%5C%2Fi%3E%2C%20153334.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.phymed.2020.153334%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.phymed.2020.153334%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6807162%26amp%3Bitem_key%3DV4UG8R6N%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Garcinone%20C%20Suppresses%20Colon%20Tumorigenesis%20Through%20the%20Gli1-dependent%20Hedgehog%20Signaling%20Pathway%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jing%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shuai%22%2C%22lastName%22%3A%22Qiu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jin%20Tae%22%2C%22lastName%22%3A%22Kim%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jae%20Seok%22%2C%22lastName%22%3A%22Cho%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ji%20Hyun%22%2C%22lastName%22%3A%22Moon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yimeng%22%2C%22lastName%22%3A%22Zhou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Joong-Hyuck%22%2C%22lastName%22%3A%22Auh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hong%20Jin%22%2C%22lastName%22%3A%22Lee%22%7D%5D%2C%22abstractNote%22%3A%22Background%5CnAlthough%20garcinone%20C%2C%20a%20natural%20xanthone%20derivative%20identified%20in%20the%20pericarp%20of%20Garcinia%20mangostana%2C%20has%20been%20demonstrated%20to%20exert%20different%20health%20beneficial%20activities%20in%20oxidative%20stress%20and%20%5Cu03b2-amyloid%20aggregation%2C%20the%20role%20of%20garcinone%20C%20in%20colon%20tumorigenesis%20has%20not%20been%20investigated.%20In%20addition%2C%20aberrant%20Hedgehog%20%28Hh%29%20signaling%20activation%20is%20associated%20with%20tumorigenesis%20including%20colon%20cancer.%20Here%2C%20we%20hypothesized%20that%20garcinone%20C%20can%20prevent%20colon%20tumorigenesis%20through%20regulating%20the%20Hh%20signaling%20pathway.%5CnMethod%5CnColony%20formation%20assay%20and%20flow%20cytometry%20were%20used%20to%20evaluate%20the%20effect%20of%20garcinone%20C%20on%20the%20proliferation%20and%20cell%20cycle%20progression%20of%20colon%20cancer%20cells.%20Protein%20expression%20of%20cell%20cycle%20related%20markers%20and%20Hh%5C%2FGli1%20signaling%20mediators%20were%20determined.%20The%20regulatory%20effect%20of%20orally%20administered%20garcinone%20C%20on%20the%20Hh%5C%2FGli1%20signaling%20pathway%20and%20colon%20tumorigenesis%20was%20evaluated%20in%20an%20azoxymethane%20%28AOM%29%5C%2Fdextran%20sulfate%20sodium%20%28DSS%29-induced%20colon%20cancer%20animal%20model.%5CnResults%5CnGarcinone%20C%20suppressed%20the%20proliferation%20of%20colon%20cancer%20cells%2C%20induced%20G0%5C%2FG1%20cell%20cycle%20arrest%2C%20as%20well%20as%20regulated%20the%20expression%20of%20cell%20cycle-related%20markers%20such%20as%20cyclin%20D1%2C%20cyclin%20E%2C%20CDK6%2C%20and%20p21.%20Garcinone%20C%20inhibited%20the%20expression%20of%20Gli1%2C%20a%20key%20mediator%20of%20Hedgehog%20signaling%2C%20and%20protein%20kinase%20B%20%28AKT%29%20phosphorylation%20in%20Smo-independent%20colon%20cancer%20cells.%20In%20the%20AOM%5C%2FDSS-induced%20colon%20tumorigenesis%20model%2C%20garcinone%20C%20significantly%20inhibited%20tumor%20development%2C%20regulated%20the%20expression%20of%20cell%20cycle%20markers%20and%20Gli1%2C%20and%20reduced%20AKT%20phosphorylation%20in%20colon%20tumor%20tissues%2C%20which%20is%20consistent%20with%20our%20in%20vitro%20results.%5CnConclusion%5CnGarcinone%20C%20can%20suppress%20colon%20tumorigenesis%20in%20vitro%20and%20in%20vivo%20through%20Gli1-dependent%20non-canonical%20Hedgehog%20signaling%2C%20suggesting%20that%20it%20may%20serve%20as%20a%20potent%20chemopreventive%20agent%20against%20colon%20tumorigenesis.%22%2C%22date%22%3A%22September%203%2C%202020%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.phymed.2020.153334%22%2C%22ISSN%22%3A%220944-7113%22%2C%22url%22%3A%22http%3A%5C%2F%5C%2Fwww.sciencedirect.com%5C%2Fscience%5C%2Farticle%5C%2Fpii%5C%2FS0944711320301665%22%2C%22deleted%22%3A1%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222020-09-03T19%3A41%3A31Z%22%7D%7D%5D%7D
Chen, J., Qiu, S., Kim, J. T., Cho, J. S., Moon, J. H., Zhou, Y., Auh, J.-H., & Lee, H. J. (2020). Garcinone C Suppresses Colon Tumorigenesis Through the Gli1-dependent Hedgehog Signaling Pathway. Phytomedicine, 153334. https://doi.org/10.1016/j.phymed.2020.153334 Cite
