Researchers investigated the effect and mechanism of β-glucan prepared from Lignosus rhinocerotis using an enzymatic method on epithelial restitution during intestinal mucosal damage.
[Journal of Ethnopharmacology]
6445212 L9346XE7 items 1 apa 0 default asc 1 160310 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-5fceb919378359ecdf330c25e52b9cbd%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22L9346XE7%22%2C%22library%22%3A%7B%22id%22%3A6445212%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Veeraperumal%20et%20al.%22%2C%22parsedDate%22%3A%222021-03-13%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EVeeraperumal%2C%20S.%2C%20Qiu%2C%20H.-M.%2C%20Tan%2C%20C.-S.%2C%20Ng%2C%20S.-T.%2C%20Zhang%2C%20W.%2C%20Tang%2C%20S.%2C%20Cheong%2C%20K.-L.%2C%20%26amp%3B%20Liu%2C%20Y.%20%282021%29.%20Restitution%20of%20epithelial%20cells%20during%20intestinal%20mucosal%20wound%20healing%3A%20the%20effect%20of%20a%20polysaccharide%20from%20the%20sclerotium%20of%20Lignosus%20rhinocerotis%20%28Cooke%29%20Ryvarden.%20%3Ci%3EJournal%20of%20Ethnopharmacology%3C%5C%2Fi%3E%2C%20114024.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jep.2021.114024%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.jep.2021.114024%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445212%26amp%3Bitem_key%3DL9346XE7%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Restitution%20of%20epithelial%20cells%20during%20intestinal%20mucosal%20wound%20healing%3A%20the%20effect%20of%20a%20polysaccharide%20from%20the%20sclerotium%20of%20Lignosus%20rhinocerotis%20%28Cooke%29%20Ryvarden%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Suresh%22%2C%22lastName%22%3A%22Veeraperumal%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hua-Mai%22%2C%22lastName%22%3A%22Qiu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chon-Seng%22%2C%22lastName%22%3A%22Tan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Szu-Ting%22%2C%22lastName%22%3A%22Ng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wancong%22%2C%22lastName%22%3A%22Zhang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shijie%22%2C%22lastName%22%3A%22Tang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kit-Leong%22%2C%22lastName%22%3A%22Cheong%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yang%22%2C%22lastName%22%3A%22Liu%22%7D%5D%2C%22abstractNote%22%3A%22Ethnopharmacological%20relevance%5CnLignosus%20rhinocerotis%20%28Cooke%29%20Ryvarden%20cultivar%20TM02%2C%20also%20known%20as%20tiger%5Cu2019s%20milk%20mushroom%2C%20is%20regarded%20as%20important%20folk%20medicine%20in%20Malaysia%2C%20while%20is%20used%20for%20the%20treatment%20of%20liver%20cancer%2C%20chronic%20hepatitis%2C%20gastric%20ulcer%20in%20traditional%20Chinese%20medicine.%20However%2C%20there%20is%20no%20compilation%20of%20scientific%20evidence%20that%20its%20protection%20for%20gastric%2C%20and%20no%20attempts%20have%20been%20made%20to%20understand%20how%20polysaccharides%20in%20Lignosus%20rhinocerotis%20might%20promote%20intestinal%20mucosal%20wound%20healing.%20Aim%20of%20the%20study%3A%20This%20study%20aimed%20to%20investigate%20the%20effect%20and%20mechanism%20of%20%5Cu03b2-glucan%20prepared%20from%20L.%20rhinocerotis%20using%20an%20enzymatic%20method%20on%20epithelial%20restitution%20during%20intestinal%20mucosal%20damage.%5CnMaterials%20and%20methods%5CnBased%20on%20FT-IR%2C%20MALDI-TOF-MS%2C%20HPSEC-MALLS-RID%2C%20and%20AFM%2C%20the%20structure%20of%20polysaccharides%20from%20L.%20rhinocerotis%20was%20analysed.%20In%20addition%2C%20polysaccharides%20were%20used%20to%20test%20for%20wound%20healing%20activity%20in%20IEC-6%20cells%20by%20measuring%20cell%20migration%2C%20proliferation%2C%20and%20expression%20of%20cell%20division%20control%20protein%2042%2C%20Rac-1%2C%20RhoA%2C%20and%20Par-3.%5CnResults%5Cn%5Cu03b2-glucan%20was%20extracted%20using%20enzyme-assisted%20extraction%2C%20and%20a%20yield%20of%20approximately%208.5%20%5Cu00b1%200.8%25%20was%20obtained%20from%20the%20dried%20biomass.%20The%20%5Cu03b2-glucan%20extracted%20by%20enzyme-assisted%20extraction%20%28EAE%29%20of%20polysaccharides%20was%20composed%20entirely%20of%20D-glucose%20with%20a%20total%20carbohydrate%20content%20of%2095.5%20%5Cu00b1%203.2%25.%20The%20results%20of%20HPLC%2C%20FTIR%2C%20and%20MALDI-TOF-MS%20analyses%20revealed%20EAEP%20to%20be%20confirmed%20as%20%5Cu03b2-glucan.%20The%20molecular%20weight%20of%20prepared%20%5Cu03b2-glucan%20was%20found%20to%20be%205.315%20%5Cu00d7%20104%20g%5C%2Fmol%20by%20HPSEC-MALLS-RID.%20Furthermore%2C%20mucosal%20wound%20healing%20studies%20showed%20that%20the%20treatment%20of%20IEC-6%20with%20a%20%5Cu03b2-glucan%20concentration%20of%20200%20%5Cu03bcg%5C%2FmL%20promoted%20cell%20migration%20and%20proliferation%2C%20and%20it%20enhanced%20the%20protein%20expression%20of%20cell%20division%20control%20protein%2042%2C%20Rac-1%2C%20RhoA%2C%20and%20Par-3.%5CnConclusions%5CnThe%20present%20study%20reveals%20that%20the%20prepared%20%5Cu03b2-glucan%20accelerates%20intestinal%20epithelial%20cell%20proliferation%20and%20migration%20via%20activation%20of%20Rho-dependent%20pathway.%20Hence%2C%20%5Cu03b2-glucan%20can%20be%20employed%20as%20a%20prospective%20therapeutic%20agent%20for%20the%20treatment%20of%20diseases%20associated%20with%20gastrointestinal%20mucosal%20damage%2C%20such%20as%20peptic%20ulcers%20and%20inflammatory%20bowel%20disease.%22%2C%22date%22%3A%22March%2013%2C%202021%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.jep.2021.114024%22%2C%22ISSN%22%3A%220378-8741%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.sciencedirect.com%5C%2Fscience%5C%2Farticle%5C%2Fpii%5C%2FS0378874121002518%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-03-19T17%3A06%3A47Z%22%7D%7D%5D%7D
Veeraperumal, S., Qiu, H.-M., Tan, C.-S., Ng, S.-T., Zhang, W., Tang, S., Cheong, K.-L., & Liu, Y. (2021). Restitution of epithelial cells during intestinal mucosal wound healing: the effect of a polysaccharide from the sclerotium of Lignosus rhinocerotis (Cooke) Ryvarden. Journal of Ethnopharmacology, 114024. https://doi.org/10.1016/j.jep.2021.114024 Cite