Anti-Proliferative and Apoptotic Effect of Gemini Curcumin in p53-Wild Type and p53-Mutant Colorectal Cancer Cell Lines

By
Justin.choi
-
0
161
The toxicity of Gemini-Cur on HT-29 and HCT116 was studied through MTT, uptake kinetics, fluorescence microscopy, annexin V/FITC, and cell cycle assays.
[International Journal of Pharmaceutics]
6445212 {6445212:BHBS3TEA} 1 apa 50 default 1 161496 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3Afalse%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22BHBS3TEA%22%2C%22library%22%3A%7B%22id%22%3A6445212%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Ebrahimi%20et%20al.%22%2C%22parsedDate%22%3A%222021-05-15%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%26lt%3Bdiv%20class%3D%26quot%3Bcsl-bib-body%26quot%3B%20style%3D%26quot%3Bline-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%26quot%3B%26gt%3B%5Cn%20%20%26lt%3Bdiv%20class%3D%26quot%3Bcsl-entry%26quot%3B%26gt%3BEbrahimi%2C%20M.%2C%20Babaei%2C%20E.%2C%20Neri%2C%20F.%2C%20%26amp%3B%20Feizi%2C%20M.%20A.%20H.%20%282021%29.%20Anti-proliferative%20and%20apoptotic%20effect%20of%20gemini%20curcumin%20in%20p53-wild%20type%20and%20p53-mutant%20colorectal%20cancer%20cell%20lines.%20%26lt%3Bi%26gt%3BInternational%20Journal%20of%20Pharmaceutics%26lt%3B%5C%2Fi%26gt%3B%2C%20%26lt%3Bi%26gt%3B601%26lt%3B%5C%2Fi%26gt%3B%2C%20120592.%20%26lt%3Ba%20class%3D%26%23039%3Bzp-DOIURL%26%23039%3B%20href%3D%26%23039%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.ijpharm.2021.120592%26%23039%3B%26gt%3Bhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.ijpharm.2021.120592%26lt%3B%5C%2Fa%26gt%3B%20%26lt%3Ba%20title%3D%26%23039%3BCite%20in%20RIS%20Format%26%23039%3B%20class%3D%26%23039%3Bzp-CiteRIS%26%23039%3B%20data-zp-cite%3D%26%23039%3Bapi_user_id%3D6445212%26amp%3Bitem_key%3DBHBS3TEA%26%23039%3B%20href%3D%26%23039%3Bjavascript%3Avoid%280%29%3B%26%23039%3B%26gt%3BCite%26lt%3B%5C%2Fa%26gt%3B%20%26lt%3B%5C%2Fdiv%26gt%3B%5Cn%26lt%3B%5C%2Fdiv%26gt%3B%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Anti-proliferative%20and%20apoptotic%20effect%20of%20gemini%20curcumin%20in%20p53-wild%20type%20and%20p53-mutant%20colorectal%20cancer%20cell%20lines%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Masoumeh%22%2C%22lastName%22%3A%22Ebrahimi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Esmaeil%22%2C%22lastName%22%3A%22Babaei%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Francesco%22%2C%22lastName%22%3A%22Neri%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mohammad%20Ali%20Hosseinpour%22%2C%22lastName%22%3A%22Feizi%22%7D%5D%2C%22abstractNote%22%3A%22Background%5CnDespite%20recent%20advances%20in%20therapy%2C%20colorectal%20cancer%20remains%20a%20leading%20cause%20of%20death%20in%20affected%20people.%20Curcumin%20is%20the%20main%20bioactive%20compound%20of%20turmeric%20that%20has%20been%20demonstrated%20as%20an%20effective%20agent%20against%20cancer.%20However%2C%20its%20poor%20stability%20and%20bioavailability%20limit%20therapeutic%20application.%20We%20previously%20showed%20that%20delivery%20of%20curcumin%20by%20using%20gemini%20surfactant%20nanoparticles%20called%20gemini%20curcumin%20%28Gemini-Cur%29%20could%20improve%20its%20solubility%2C%20uptake%20and%20toxic%20effect%20on%20breast%20and%20ovarian%20cancer%20cells.%20Here%2C%20we%20aimed%20to%20investigate%20the%20anticancer%20activity%20of%20Gemini-Cur%20in%20both%20p53-mutant%20and%20p53-wild%20type%20colorectal%20cancer%20cells.%20The%20toxicity%20of%20Gemini-Cur%20on%20HT-29%20and%20HCT116%20was%20studied%20through%20MTT%2C%20uptake%20kinetics%2C%20fluorescence%20microscopy%2C%20annexin%20V%5C%2FFITC%2C%20and%20cell%20cycle%20assays.%20Also%2C%20real-time%20PCR%20and%20western%20blotting%20were%20performed%20to%20evaluate%20the%20expression%20of%20p53%2C%20p21%2C%20BAX%2C%20BCL-2%2C%20and%20NOXA%20genes.%20Our%20data%20showed%20that%20Gemini-Cur%20not%20only%20enters%20cells%20quite%20rapidly%20compared%20to%20free%20curcumin%20crystals%2C%20but%20also%20suppresses%20HT-29%20and%20HCT-116%20cells%20proliferation%20in%20a%20time-%20and%20dose-dependent%20manner%20%28p%5Cu00a0%26lt%3B%5Cu00a00.001%29.%20The%20IC50%20values%20as%20well%20as%20apoptosis%20assays%20showed%20that%20p53-wild%20type%20cells%20are%20sensitive%20to%20Gemini-Cur.%20Flow%20cytometry%20also%20revealed%20that%20the%20number%20of%20apoptotic%20cells%20is%20dramatically%20increased%20in%20HCT-116%20cells%20earlier%20than%20HT-29%20cells%20%28p%5Cu00a0%26lt%3B%5Cu00a00.0001%29.%20Gemini-Cur%20upregulated%20apoptotic%20genes%20including%20p53%20%28in%20both%20mutant%20and%20wild-type%20forms%29%2C%20p21%2C%20NOXA%20and%20BAX%20while%20decreased%20anti-apoptotic%20BCL-2%20in%20mRNA%20and%20protein%20level%20%28p%5Cu00a0%26lt%3B%5Cu00a00.0001%29.%20As%20a%20hallmark%20of%20apoptosis%2C%20the%20expression%20ratio%20of%20BAX%5C%2FBCL-2%20was%20significantly%20increased%20in%20all%20treated%20cells.%20Taken%20together%2C%20our%20findings%20demonstrated%20that%20Gemini-Cur%20suppresses%20the%20proliferation%20of%20cancer%20cells%20via%20induction%20of%20apoptosis%20and%20could%20be%20considered%20as%20novel%20nano-formulated%20phytochemical%20for%20cancer%20targeting.%22%2C%22date%22%3A%22May%2015%2C%202021%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.ijpharm.2021.120592%22%2C%22ISSN%22%3A%220378-5173%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.sciencedirect.com%5C%2Fscience%5C%2Farticle%5C%2Fpii%5C%2FS0378517321003975%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-04-23T17%3A40%3A13Z%22%7D%7D%5D%7D
Ebrahimi, M., Babaei, E., Neri, F., & Feizi, M. A. H. (2021). Anti-proliferative and apoptotic effect of gemini curcumin in p53-wild type and p53-mutant colorectal cancer cell lines. International Journal of Pharmaceutics, 601, 120592. https://doi.org/10.1016/j.ijpharm.2021.120592 Cite
AbstractGraphical Abstract