Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsTo reveal the trajectories of smooth muscle cell (SMC) transdifferentiation during atherosclerosis and to identify molecular targets for disease therapy, researchers combined SMC fate mapping and single-cell RNA sequencing of both mouse and human atherosclerotic plaques.
[Circulation]
1254621 {1254621:Q2ZDY6S3} apa 50 1 154431 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-e5c2eb61c19722f2d4c180c6f49d4478%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22Q2ZDY6S3%22%2C%22library%22%3A%7B%22id%22%3A1254621%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Pan%20Huize%20et%20al.%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EPan%20Huize%2C%20Xue%20Chenyi%2C%20Auerbach%20Benjamin%20J.%2C%20Fan%20Jiaxin%2C%20Bashore%20Alexander%20C.%2C%20Cui%20Jian%2C%20Yang%20Dina%20Y.%2C%20Trignano%20Sarah%20B.%2C%20Liu%20Wen%2C%20Shi%20Jianting%2C%20Ihuegbu%20Chinyere%20O.%2C%20Bush%20Erin%20C.%2C%20Worley%20Jeremy%2C%20Vlahos%20Lukas%2C%20Laise%20Pasquale%2C%20Solomon%20Robert%20A.%2C%20Connolly%20Edward%20S.%2C%20Califano%20Andrea%2C%20Sims%20Peter%20A.%2C%20%26%23x2026%3B%20Reilly%20Muredach%20P.%20%28n.d.%29.%20Single-Cell%20Genomics%20Reveals%20a%20Novel%20Cell%20State%20During%20Smooth%20Muscle%20Cell%20Phenotypic%20Switching%20and%20Potential%20Therapeutic%20Targets%20for%20Atherosclerosis%20in%20Mouse%20and%20Human.%20%3Ci%3ECirculation%3C%5C%2Fi%3E%2C%20%3Ci%3E0%3C%5C%2Fi%3E%280%29.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1161%5C%2FCIRCULATIONAHA.120.048378%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1161%5C%2FCIRCULATIONAHA.120.048378%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D1254621%26amp%3Bitem_key%3DQ2ZDY6S3%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Single-Cell%20Genomics%20Reveals%20a%20Novel%20Cell%20State%20During%20Smooth%20Muscle%20Cell%20Phenotypic%20Switching%20and%20Potential%20Therapeutic%20Targets%20for%20Atherosclerosis%20in%20Mouse%20and%20Human%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Pan%20Huize%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Xue%20Chenyi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Auerbach%20Benjamin%20J.%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Fan%20Jiaxin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Bashore%20Alexander%20C.%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Cui%20Jian%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Yang%20Dina%20Y.%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Trignano%20Sarah%20B.%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Liu%20Wen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Shi%20Jianting%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Ihuegbu%20Chinyere%20O.%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Bush%20Erin%20C.%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Worley%20Jeremy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Vlahos%20Lukas%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Laise%20Pasquale%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Solomon%20Robert%20A.%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Connolly%20Edward%20S.%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Califano%20Andrea%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Sims%20Peter%20A.%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Zhang%20Hanrui%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Li%20Mingyao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22name%22%3A%22Reilly%20Muredach%20P.%22%7D%5D%2C%22abstractNote%22%3A%22Background%3A%20Smooth%20muscle%20cells%20%28SMC%29%20play%20significant%20roles%20in%20atherosclerosis%20via%20phenotypic%20switching%2C%20a%20pathological%20process%20in%20which%20SMC%20dedifferentiation%2C%20migration%20and%20transdifferentiation%20into%20other%20cell%20types.%20Yet%2C%20how%20SMC%20contribute%20to%20pathophysiology%20of%20atherosclerosis%20remains%20elusive.%20%5CnMethods%3A%20To%20reveal%20the%20trajectories%20of%20SMC%20transdifferentiation%20during%20atherosclerosis%20and%20to%20identify%20molecular%20targets%20for%20disease%20therapy%2C%20we%20combined%20SMC%20fate%20mapping%20and%20single-cell%20RNA%20sequencing%20of%20both%20mouse%20and%20human%20atherosclerotic%20plaques.%20We%20also%20performed%20cell%20biology%20experiments%20on%20isolated%20SMC-derived%20cells%2C%20conducted%20integrative%20human%20genomics%2C%20and%20employed%20pharmacological%20studies%20targeting%20SMC-derived%20cells%20both%20in%20vivo%20and%20in%20vitro.%20%5CnResults%3A%20We%20found%20that%20SMC%20transitioned%20to%20an%20intermediate%20cell%20state%20during%20atherosclerosis%2C%20which%20was%20also%20found%20in%20human%20atherosclerotic%20plaques%20of%20carotid%20and%20coronary%20arteries.%20SMC-derived%20intermediate%20cells%2C%20termed%20%5C%22SEM%5C%22%20cells%2C%20were%20multipotent%20and%20could%20differentiate%20into%20macrophage-like%20and%20fibrochondrocyte-like%20cells%2C%20as%20well%20as%20return%20towards%20SMC%20phenotype.%20Retinoic%20acid%20%28RA%29%20signaling%20was%20identified%20as%20a%20regulator%20of%20SMC%20to%20SEM%20cell%20transition%20and%20RA%20signaling%20was%20dysregulated%20in%20symptomatic%20human%20atherosclerosis.%20Human%20genomics%20revealed%20enrichment%20of%20genome%20wide%20association%20study%20%28GWAS%29%20signals%20for%20coronary%20artery%20disease%20%28CAD%29%20in%20RA%20signaling%20target%20gene%20loci%20and%20correlation%20between%20CAD%20risk%20alleles%20and%20repressed%20expression%20of%20these%20genes.%20Activation%20of%20RA%20signaling%20by%20all-trans%20retinoic%20acid%20%28ATRA%29%2C%20an%20anti-cancer%20drug%20for%20acute%20promyelocytic%20leukemia%2C%20blocked%20SMC%20transition%20to%20SEM%20cells%2C%20reduced%20atherosclerotic%20burden%20and%20promoted%20fibrous%20cap%20stability.%5CnConclusions%3A%20Integration%20of%20cell-specific%20fate%20mapping%2C%20single-cell%20genomics%20and%20human%20genetics%20adds%20novel%20insights%20into%20the%20complexity%20of%20SMC%20biology%20and%20reveals%20regulatory%20pathways%20for%20therapeutic%20targeting%20of%20SMC%20transitions%20in%20atherosclerotic%20cardiovascular%20disease.%22%2C%22date%22%3A%22%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1161%5C%2FCIRCULATIONAHA.120.048378%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.ahajournals.org%5C%2Fdoi%5C%2F10.1161%5C%2FCIRCULATIONAHA.120.048378%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222020-09-23T17%3A29%3A50Z%22%7D%7D%5D%7D
Pan Huize, Xue Chenyi, Auerbach Benjamin J., Fan Jiaxin, Bashore Alexander C., Cui Jian, Yang Dina Y., Trignano Sarah B., Liu Wen, Shi Jianting, Ihuegbu Chinyere O., Bush Erin C., Worley Jeremy, Vlahos Lukas, Laise Pasquale, Solomon Robert A., Connolly Edward S., Califano Andrea, Sims Peter A., … Reilly Muredach P. (n.d.). Single-Cell Genomics Reveals a Novel Cell State During Smooth Muscle Cell Phenotypic Switching and Potential Therapeutic Targets for Atherosclerosis in Mouse and Human. Circulation, 0(0). https://doi.org/10.1161/CIRCULATIONAHA.120.048378 Cite
