Cancer Stem Cell News
Cell Therapy News
Dermal Cell News
Endothelial Cell News
ESC & iPSC News
Extracellular Matrix News
Hematopoiesis News
Hepatic Cell News
Human Immunology News
Immune Regulation News
Intestinal Cell News
Mammary Cell News
Mesenchymal Cell News
Muscle Cell News
Neural Cell News
Organoid News
Pancreatic Cell News
Prostate Cell News
Pulmonary Cell NewsResearchers evaluated single cell photonics as a discriminator of cell phenotype in vitro before the presence of vascular stem cells within vascular lesions.
[Stem Cell Reviews and Reports]
6445218 7ZY88NBB items 1 apa 0 default asc 1 160267 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-2bb05741a7358583fb896ccd18f4b27f%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%227ZY88NBB%22%2C%22library%22%3A%7B%22id%22%3A6445218%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Molony%20et%20al.%22%2C%22parsedDate%22%3A%222021-03-17%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EMolony%2C%20C.%2C%20King%2C%20D.%2C%20Di%20Luca%2C%20M.%2C%20Kitching%2C%20M.%2C%20Olayinka%2C%20A.%2C%20Hakimjavadi%2C%20R.%2C%20Julius%2C%20L.%20A.%20N.%2C%20Fitzpatrick%2C%20E.%2C%20Gusti%2C%20Y.%2C%20Burtenshaw%2C%20D.%2C%20Healy%2C%20K.%2C%20Finlay%2C%20E.%20K.%2C%20Kernan%2C%20D.%2C%20Llobera%2C%20A.%2C%20Liu%2C%20W.%2C%20Morrow%2C%20D.%2C%20Redmond%2C%20E.%20M.%2C%20Ducr%26%23xE9%3Be%2C%20J.%2C%20%26amp%3B%20Cahill%2C%20P.%20A.%20%282021%29.%20Disease-Relevant%20Single%20Cell%20Photonic%20Signatures%20Identify%20S100%26%23x3B2%3B%20Stem%20Cells%20and%20their%20Myogenic%20Progeny%20in%20Vascular%20Lesions.%20%3Ci%3EStem%20Cell%20Reviews%20and%20Reports%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs12015-021-10125-x%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs12015-021-10125-x%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445218%26amp%3Bitem_key%3D7ZY88NBB%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Disease-Relevant%20Single%20Cell%20Photonic%20Signatures%20Identify%20S100%5Cu03b2%20Stem%20Cells%20and%20their%20Myogenic%20Progeny%20in%20Vascular%20Lesions%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Claire%22%2C%22lastName%22%3A%22Molony%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Damien%22%2C%22lastName%22%3A%22King%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mariana%22%2C%22lastName%22%3A%22Di%20Luca%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Michael%22%2C%22lastName%22%3A%22Kitching%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Abidemi%22%2C%22lastName%22%3A%22Olayinka%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Roya%22%2C%22lastName%22%3A%22Hakimjavadi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lourdes%20A.%20N.%22%2C%22lastName%22%3A%22Julius%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emma%22%2C%22lastName%22%3A%22Fitzpatrick%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yusof%22%2C%22lastName%22%3A%22Gusti%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Denise%22%2C%22lastName%22%3A%22Burtenshaw%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Killian%22%2C%22lastName%22%3A%22Healy%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Emma%20K.%22%2C%22lastName%22%3A%22Finlay%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Kernan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Andreu%22%2C%22lastName%22%3A%22Llobera%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Weimin%22%2C%22lastName%22%3A%22Liu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22David%22%2C%22lastName%22%3A%22Morrow%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eileen%20M.%22%2C%22lastName%22%3A%22Redmond%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jens%22%2C%22lastName%22%3A%22Ducr%5Cu00e9e%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Paul%20A.%22%2C%22lastName%22%3A%22Cahill%22%7D%5D%2C%22abstractNote%22%3A%22A%20hallmark%20of%20subclinical%20atherosclerosis%20is%20the%20accumulation%20of%20vascular%20smooth%20muscle%20cell%20%28SMC%29-like%20cells%20leading%20to%20intimal%20thickening%20and%20lesion%20formation.%20While%20medial%20SMCs%20contribute%20to%20vascular%20lesions%2C%20the%20involvement%20of%20resident%20vascular%20stem%20cells%20%28vSCs%29%20remains%20unclear.%20We%20evaluated%20single%20cell%20photonics%20as%20a%20discriminator%20of%20cell%20phenotype%20in%20vitro%20before%20the%20presence%20of%20vSC%20within%20vascular%20lesions%20was%20assessed%20ex%20vivo%20using%20supervised%20machine%20learning%20and%20further%20validated%20using%20lineage%20tracing%20analysis.%20Using%20a%20novel%20lab-on-a-Disk%28Load%29%20platform%2C%20label-free%20single%20cell%20photonic%20emissions%20from%20normal%20and%20injured%20vessels%20ex%20vivo%20were%20interrogated%20and%20compared%20to%20freshly%20isolated%20aortic%20SMCs%2C%20cultured%20Movas%20SMCs%2C%20macrophages%2C%20B-cells%2C%20S100%5Cu03b2%2B%20mVSc%2C%20bone%20marrow%20derived%20mesenchymal%20stem%20cells%20%28MSC%29%20and%20their%20respective%20myogenic%20progeny%20across%20five%20broadband%20light%20wavelengths%20%28%5Cu03bb465%20-%20%5Cu03bb670%20%5Cu00b1%2020%5Cu00a0nm%29.%20We%20found%20that%20profiles%20were%20of%20sufficient%20coverage%2C%20specificity%2C%20and%20quality%20to%20clearly%20distinguish%20medial%20SMCs%20from%20different%20vascular%20beds%20%28carotid%20vs%20aorta%29%2C%20discriminate%20normal%20carotid%20medial%20SMCs%20from%20lesional%20SMC-like%20cells%20ex%20vivo%20following%20flow%20restriction%2C%20and%20identify%20SMC%20differentiation%20of%20a%20series%20of%20multipotent%20stem%20cells%20following%20treatment%20with%20transforming%20growth%20factor%20beta%201%20%28TGF-%20%5Cu03b21%29%2C%20the%20Notch%20ligand%20Jagged1%2C%20and%20Sonic%20Hedgehog%20using%20multivariate%20analysis%2C%20in%20part%2C%20due%20to%20photonic%20emissions%20from%20enhanced%20collagen%20III%20and%20elastin%20expression.%20Supervised%20machine%20learning%20supported%20genetic%20lineage%20tracing%20analysis%20of%20S100%5Cu03b2%2B%20vSCs%20and%20identified%20the%20presence%20of%20S100%5Cu03b2%2BvSC-derived%20myogenic%20progeny%20within%20vascular%20lesions.%20We%20conclude%20disease-relevant%20photonic%20signatures%20may%20have%20predictive%20value%20for%20vascular%20disease.%22%2C%22date%22%3A%222021-03-17%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1007%5C%2Fs12015-021-10125-x%22%2C%22ISSN%22%3A%222629-3277%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs12015-021-10125-x%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-03-18T23%3A27%3A57Z%22%7D%7D%5D%7D
Molony, C., King, D., Di Luca, M., Kitching, M., Olayinka, A., Hakimjavadi, R., Julius, L. A. N., Fitzpatrick, E., Gusti, Y., Burtenshaw, D., Healy, K., Finlay, E. K., Kernan, D., Llobera, A., Liu, W., Morrow, D., Redmond, E. M., Ducrée, J., & Cahill, P. A. (2021). Disease-Relevant Single Cell Photonic Signatures Identify S100β Stem Cells and their Myogenic Progeny in Vascular Lesions. Stem Cell Reviews and Reports. https://doi.org/10.1007/s12015-021-10125-x Cite
