An Epigenetic GPI Anchor Defect Impairs TLR4 Signaling in the B Cell Transdifferentiation Model for Primary Human Monocytes BLaER1

The authors identified an important caveat when investigating TLR4-driven signaling in BLaER1 cells. They showed that this model contained glycosylphosphatidylinositol anchor-deficient cells, which lacked CD14 surface expression when differentiated to monocytes, resulting in diminished LPS/TLR4 but not TLR7/TLR8 responsiveness.