An ovarian cancer cell line, PEO4, was initially reprogrammed into induced pluripotent stem cells using the classical four factors OCT4, SOX2, KLF4 and MYC using lentivirus transduction.
[International Journal of Biochemistry & Cell Biology]
6445212 P3TCWD6S items 1 apa 0 default asc 1 165329 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-165b25b9d4428627602d99ed48c83930%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22P3TCWD6S%22%2C%22library%22%3A%7B%22id%22%3A6445212%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Bindhya%20et%20al.%22%2C%22parsedDate%22%3A%222021-07-31%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBindhya%2C%20S.%2C%20Sidhanth%2C%20C.%2C%20Krishnapriya%2C%20S.%2C%20Garg%2C%20M.%2C%20%26amp%3B%20Ganesan%2C%20T.%20S.%20%282021%29.%20Development%20and%20in%20vitro%20characterisation%20of%20an%20induced%20pluripotent%20stem%20cell%20model%20of%20ovarian%20cancer.%20%3Ci%3EThe%20International%20Journal%20of%20Biochemistry%20%26amp%3B%20Cell%20Biology%3C%5C%2Fi%3E%2C%20106051.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.biocel.2021.106051%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.biocel.2021.106051%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445212%26amp%3Bitem_key%3DP3TCWD6S%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Development%20and%20in%20vitro%20characterisation%20of%20an%20induced%20pluripotent%20stem%20cell%20model%20of%20ovarian%20cancer%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Bindhya%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22C.%22%2C%22lastName%22%3A%22Sidhanth%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22S.%22%2C%22lastName%22%3A%22Krishnapriya%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Manoj%22%2C%22lastName%22%3A%22Garg%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22T%20S%22%2C%22lastName%22%3A%22Ganesan%22%7D%5D%2C%22abstractNote%22%3A%22Ovarian%20cancer%20recurs%20despite%20advances%20in%20treatment%20and%20is%20due%20to%20drug%20resistance.%20The%20persistence%20of%20cancer%20stem%20cells%20%28CSCs%29%20is%20one%20of%20the%20causes.%20It%20has%20been%20challenging%20to%20maintain%20CSCs%20long%20term%20in%20culture%20from%20primary%20malignant%20cells.%20Reprogramming%20cancer%20cells%20into%20induced%20pluripotent%20stem%20cells%20%28iPSCs%29%20could%20be%20an%20approach%20to%20achieve%20this.%20An%20ovarian%20cancer%20cell%20line%2C%20PEO4%2C%20was%20initially%20reprogrammed%20into%20iPSCs%20using%20the%20classical%20four%20factors%20OCT4%2C%20SOX2%2C%20KLF4%20and%20MYC%20%28OSKM%29%20using%20lentivirus%20transduction.%20The%20PEO4-OSKM-cells%20had%20all%20the%20hallmarks%20of%20iPSCs.%20As%20MYC%20is%20oncogenic%2C%20we%20have%20replaced%20it%20with%20GLIS1%20and%20show%20that%20PEO4%20cells%20could%20be%20transformed%20into%20iPSCs.%20The%20transfection%20efficiency%20was%20two-fold%20better%20with%20OCT4-SOX2-KLF4-GLIS1%20%28OSKG%29%20with%20larger%20colonies.%20Further%2C%20normal%20fallopian%20tube%20epithelial%20cells%20were%20also%20transformed%20using%20OSKG%20into%20iPSCs.%20iPSCs%20expressed%20CSCs%20markers%20such%20as%20CD133%2C%20EPHA1%2C%20ALDH1A1%20and%20LGR5%20prominently%20and%20were%20more%20resistant%20to%20cisplatin%20and%20taxol%20as%20compared%20to%20parental%20PEO4%20cells.%20PEO4-OSKM-iPSCs%20cells%20formed%20more%20colonies%20in%20a%20clonogenic%20assay%20as%20compared%20to%20PEO4-OSKG-iPSCs%20and%20parental%20cells.%20These%20results%20provide%20a%20first%20insight%20that%20both%20an%20ovarian%20cancer%20cell%20line%20and%20fallopian%20tube%20epithelial%20cells%20can%20be%20reprogrammed%20and%20GLIS1%20can%20successfully%20replace%20MYC%20as%20a%20transcription%20factor.%20This%20in%20vitro%20model%20is%20useful%20for%20future%20experiments%20to%20understand%20the%20characteristics%20of%20CSCs%20in%20the%20pathogenesis%20of%20ovarian%20cancer.%22%2C%22date%22%3A%22July%2031%2C%202021%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.biocel.2021.106051%22%2C%22ISSN%22%3A%221357-2725%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.sciencedirect.com%5C%2Fscience%5C%2Farticle%5C%2Fpii%5C%2FS135727252100131X%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-08-04T20%3A11%3A48Z%22%7D%7D%5D%7D
Bindhya, S., Sidhanth, C., Krishnapriya, S., Garg, M., & Ganesan, T. S. (2021). Development and in vitro characterisation of an induced pluripotent stem cell model of ovarian cancer. The International Journal of Biochemistry & Cell Biology, 106051. https://doi.org/10.1016/j.biocel.2021.106051 Cite