Researchers investigatef whether Cisplatin, Imatinib, and 5-Fluorouracil alter the tumoroid growth of metastatic colorectal cancer.
[Cells]
6445218 {6445218:UDNLWM9P} apa 50 1 158970 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-169ac7aa0aa2d245b2608e93862eb504%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22UDNLWM9P%22%2C%22library%22%3A%7B%22id%22%3A6445218%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Sogawa%20et%20al.%22%2C%22parsedDate%22%3A%222021-02%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ESogawa%2C%20C.%2C%20Eguchi%2C%20T.%2C%20Namba%2C%20Y.%2C%20Okusha%2C%20Y.%2C%20Aoyama%2C%20E.%2C%20Ohyama%2C%20K.%2C%20%26amp%3B%20Okamoto%2C%20K.%20%282021%29.%20Gel-Free%203D%20Tumoroids%20with%20Stem%20Cell%20Properties%20Modeling%20Drug%20Resistance%20to%20Cisplatin%20and%20Imatinib%20in%20Metastatic%20Colorectal%20Cancer.%20%3Ci%3ECells%3C%5C%2Fi%3E%2C%20%3Ci%3E10%3C%5C%2Fi%3E%282%29%2C%20344.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fcells10020344%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.3390%5C%2Fcells10020344%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445218%26amp%3Bitem_key%3DUDNLWM9P%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Gel-Free%203D%20Tumoroids%20with%20Stem%20Cell%20Properties%20Modeling%20Drug%20Resistance%20to%20Cisplatin%20and%20Imatinib%20in%20Metastatic%20Colorectal%20Cancer%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Chiharu%22%2C%22lastName%22%3A%22Sogawa%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Takanori%22%2C%22lastName%22%3A%22Eguchi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yuri%22%2C%22lastName%22%3A%22Namba%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yuka%22%2C%22lastName%22%3A%22Okusha%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eriko%22%2C%22lastName%22%3A%22Aoyama%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kazumi%22%2C%22lastName%22%3A%22Ohyama%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kuniaki%22%2C%22lastName%22%3A%22Okamoto%22%7D%5D%2C%22abstractNote%22%3A%22Researchers%20have%20developed%20several%20three-dimensional%20%283D%29%20culture%20systems%2C%20including%20spheroids%2C%20organoids%2C%20and%20tumoroids%20with%20increased%20properties%20of%20cancer%20stem%20cells%20%28CSCs%29%2C%20also%20called%20cancer-initiating%20cells%20%28CICs%29.%20Drug%20resistance%20is%20a%20crucial%20issue%20involving%20recurrence%20in%20cancer%20patients.%20Many%20studies%20on%20anti-cancer%20drugs%20have%20been%20reported%20using%202D%20culture%20systems%2C%20whereas%203D%20cultured%20tumoroids%20have%20many%20advantages%20for%20assessing%20drug%20sensitivity%20and%20resistance.%20Here%2C%20we%20aimed%20to%20investigate%20whether%20Cisplatin%20%28a%20DNA%20crosslinker%29%2C%20Imatinib%20%28a%20multiple%20tyrosine%20kinase%20inhibitor%29%2C%20and%205-Fluorouracil%20%285-FU%3A%20an%20antimetabolite%29%20alter%20the%20tumoroid%20growth%20of%20metastatic%20colorectal%20cancer%20%28mCRC%29.%20Gene%20expression%20signatures%20of%20highly%20metastatic%20aggregative%20CRC%20%28LuM1%20cells%29%20vs.%20low-metastatic%2C%20non-aggregative%20CRC%20%28Colon26%20and%20NM11%20cells%29%20were%20analyzed%20using%20microarray.%20To%20establish%20a%203D%20culture-based%20multiplexing%20reporter%20assay%20system%2C%20LuM1%20was%20stably%20transfected%20with%20the%20Mmp9%20promoter-driven%20ZsGreen%20fluorescence%20reporter%20gene%2C%20which%20was%20designated%20as%20LuM1%5C%2Fm9%20cells%20and%20cultured%20in%20NanoCulture%20Plate%5Cu00ae%2C%20a%20gel-free%203D%20culture%20device.%20LuM1%20cells%20highly%20expressed%20mRNA%20encoding%20ABCG2%20%28a%20drug%20resistance%20pump%2C%20i.e.%2C%20CSC%5C%2FCIC%20marker%29%2C%20other%20CSC%5C%2FCIC%20markers%20%28DLL1%2C%20EpCAM%2C%20podoplanin%2C%20STAT3%5C%2F5%29%2C%20pluripotent%20stem%20cell%20markers%20%28Sox4%5C%2F7%2C%20N-myc%2C%20GATA3%2C%20Nanog%29%2C%20and%20metastatic%20markers%20%28MMPs%2C%20Integrins%2C%20EGFR%29%2C%20compared%20to%20the%20other%20two%20cell%20types.%20Hoechst%20efflux%20stem%20cell-like%20side%20population%20was%20increased%20in%20LuM1%20%287.8%25%29%20compared%20with%20Colon26%20%282.9%25%29%2C%20both%20of%20which%20were%20markedly%20reduced%20by%20verapamil%20treatment%2C%20an%20ABCG2%20inhibitor.%20Smaller%20cell%20aggregates%20of%20LuM1%20were%20more%20sensitive%20to%20Cisplatin%20%28at%2010%20%5Cu03bcM%29%2C%20whereas%20larger%20tumoroids%20with%20increased%20ABCG2%20expression%20were%20insensitive.%20Notably%2C%20Cisplatin%20%282%20%5Cu03bcM%29%20and%20Imatinib%20%2810%20%5Cu03bcM%29%20at%20low%20concentrations%20significantly%20promoted%20tumoroid%20formation%20%28cell%20aggregation%29%20and%20increased%20Mmp9%20promoter%20activity%20in%20mCRC%20LuM1%5C%2Fm9%2C%20while%20not%20cytotoxic%20to%20them.%20On%20the%20other%20hand%2C%205-FU%20significantly%20inhibited%20tumoroid%20growth%2C%20although%20not%20completely.%20Thus%2C%20drug%20resistance%20in%20cancer%20with%20increased%20stem%20cell%20properties%20was%20modeled%20using%20the%20gel-free%203D%20cultured%20tumoroid%20system.%20The%20tumoroid%20culture%20is%20useful%20and%20easily%20accessible%20for%20the%20assessment%20of%20drug%20sensitivity%20and%20resistance.%22%2C%22date%22%3A%222021%5C%2F2%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.3390%5C%2Fcells10020344%22%2C%22ISSN%22%3A%22%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.mdpi.com%5C%2F2073-4409%5C%2F10%5C%2F2%5C%2F344%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-02-10T23%3A54%3A31Z%22%7D%7D%5D%7D
Sogawa, C., Eguchi, T., Namba, Y., Okusha, Y., Aoyama, E., Ohyama, K., & Okamoto, K. (2021). Gel-Free 3D Tumoroids with Stem Cell Properties Modeling Drug Resistance to Cisplatin and Imatinib in Metastatic Colorectal Cancer. Cells, 10(2), 344. https://doi.org/10.3390/cells10020344 Cite